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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
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Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
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Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Santa ines. |
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Thesagro: |
Ovis Aries. |
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Thesaurus Nal: |
cryopreservation; gene expression. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02970naa a2200289 a 4500
001 2085659
005 2023-01-27
008 2018 bl uuuu u00u1 u #d
100 1 $aSARAIVA, H. F. R. de A.
245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource]
260 $c2018
520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $acryopreservation
650 $agene expression
650 $aOvis Aries
653 $aIn vivo embryo production
653 $aSanta ines
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDAO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 22 | |
| 4. |  | MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G. de; RANGEL, P. S. C.; CÔRTES, L. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Estrus induction in the non-breeding season is not associated with hydrometra in dairy goats. Research, Society and Development, v. 10, n. 11, e21101119162, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | TEIXEIRA, T. A.; FONSECA, J. F. da; SOUZA-FABJAN, J. M. G. de; CARVALHEIRA, L. de R.; FERNANDES, D. A. de M.; BRANDÃO, F. Z. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health and Production, Heidelberg, v. 48, n. 3, p. 545-551, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | SOUZA-FABJAN, J. M. G. de; OLIVARES, C. C. S.; VELTRI, A. B. P.; BALARO, M. F. A.; FREITAS, V. J. de F.; FONSECA, J. F. da; BRANDÃO, F. Z. Avaliação da longevidade espermática após o uso de diferentes métodos de seleção de espermatozóides ovinos congelados. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 199.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | CORTAT, P. R.; DIAS, M. B.; PAULA, C. J. C. de; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da. Correlation between estrus onset and ovarian parameters in dairy goats. Ciência Animal Brasileira, v. 23, e-70813E, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. Comparison of different sperm selection techniques in ram frozen-thawed sperm. Acta Scientiae Veterinariae, v. 45, p. 1431, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | BRAGANÇA, G. M.; ALFRADIQUE, V. A. P.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; ARASHIRO, E. K. N.; BALARO, M. F. A.; OLIVEIRA, I. C. de; BRANDÃO, F. Z.; CÔRTES, L. R.; FONSECA, J. F. da. Efeito de diferentes protocolos hormonais para superestimulação ovariana sobre o número e a qualidade de oócitos em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 470, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | VERGANI, G. B.; FONSECA, J. F. da; TREVIZAN, J. T.; PEREIRA, V. S. do A.; GARCIA, A. R.; ESTEVES, S. N.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G. de; OLIVEIRA, M. E. F. Effects of hCG administered 7.5 days after synchronous estrus induction during the non-breading season in Morada Nova ewes. Animal Reproduction, v.17, n.3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | VERGANI, G. B.; FONSECA, J. F. da; TREVIZAN, J. T.; PEREIRA, V. S. do A.; GARCIA, A. R.; ESTEVES, S. N.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G. de; OLIVEIRA, M. E. F. Effects of hCG administered 7.5 days after synchronous estrus induction during the non-breading season in Morada Nova ewes. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | BRAGANÇA, G. M.; BALARO, M. F. A.; FONSECA, J. F. da; PINTO, P. H. N.; ROSA, R. M. da; RIBEIRO, L. S.; ALMEIDA, M. S.; SOUZA FABJAN, J. M. G. de; GARCIA, A. R.; BRANDÃO, F. Z. Ultrassonografia doppler no diagnóstico precoce da gestação em ovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, v. 30, Foz do Iguaçu, 2016. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. p. 292. Resumo SBTE 175.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | TAIRA, A. R.; SANTOS, J. D. R.; COSENTINO, I. O.; BRAIR, V. L.; ESPÍRITO SANTO, C. G. do; PINTO, P. H. N.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, J. Z. The use of exogenous progesterone in sheep superovulation protocols influences the percentage of viable embryos. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDXI expression in in vivo-produced ovine embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 901.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology, v. 105, p. 150-157, Jan. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. Theriogenology, v. 105, p. 150-157, jan. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 22 | |
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